5(6)-Aminofluorescein CAS 27599-63-9

5-Aminofluorescein is a fluorescent labeling reagent commonly used in biochemical research. Due to the presence of a reactive amino group, it can serve as an active moiety that covalently binds to proteins, antibodies, peptides, or nucleic acids through chemical reactions, creating fluorescently labeled biomolecular probes. These labeled molecules allow for the visualization of intracellular distribution via fluorescence microscopy or the analysis of cell-surface marker expression levels using flow cytometry. For instance, antibodies labeled with 5-aminofluorescein can be employed in immunofluorescence staining, enabling scientists to observe the localization and expression of specific intracellular proteins. Furthermore, leveraging its fluorescent properties, researchers can design various enzyme activity assay kits that monitor enzyme activity in real-time by detecting fluorescence signal changes resulting from enzyme-catalyzed reactions.

In the medical field, 5-aminofluorescein and its derivatives show promise for tumor diagnosis and surgical navigation. Novel probes—such as 5-aminofluorescein-labeled human serum albumin (5-AF-HSA)—are currently under investigation, particularly for the treatment of gliomas. These probes can specifically accumulate in malignant brain tumor tissues; through fluorescence imaging, they assist surgeons in clearly delineating tumor margins during operations, thereby facilitating more complete tumor resection. Although 5-aminolevulinic acid (5-ALA) is the drug currently in common clinical use, 5-aminofluorescein—as a direct fluorescent dye—offers a new tool for precision oncology when subjected to appropriate chemical modification.

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